The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 31, 2020
Filed:
Jun. 19, 2017
Applicant:
Portola Pharmaceuticals, Inc., South San Francisco, CA (US);
Inventors:
Mark Karbarz, South San Francisco, CA (US);
Pamela B. Conley, Palo Alto, CA (US);
Genmin Lu, Burlingame, CA (US);
Assignee:
Portola Pharmaceuticals, Inc., South San Francisco, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 9/64 (2006.01); C07K 1/18 (2006.01); C07K 1/20 (2006.01); C07K 1/22 (2006.01);
U.S. Cl.
CPC ...
C12N 9/6432 (2013.01); C07K 1/18 (2013.01); C07K 1/20 (2013.01); C07K 1/22 (2013.01);
Abstract
The present disclosure provides methods for manufacturing a fXa derivative protein at large scale leading to high yield of highly pure protein product. The method may include adding a detergent to a sample that contains a polynucleotide construct encoding the protein and purifying the protein through a soybean trypsin inhibitor (STI)-based affinity chromatograph, an ion exchange and mixed mode chromatograph and a hydrophobic interaction.