The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 31, 2020

Filed:

Apr. 27, 2017
Applicant:

Cellular Dynamics International, Inc., Madison, WI (US);

Inventors:

Casey Stankewicz, Madison, WI (US);

Matt Riley, Madison, WI (US);

Nathaniel Beardsley, Oregon, WI (US);

Wen Bo Wang, Waunakee, WI (US);

Peter Fuhrken, Madison, WI (US);

Steven Kattman, Madison, WI (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/077 (2010.01); C12N 5/0735 (2010.01); A61K 48/00 (2006.01); C07K 14/50 (2006.01); C07H 21/04 (2006.01); C07K 14/475 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0657 (2013.01); A61K 48/00 (2013.01); C07H 21/04 (2013.01); C07K 14/475 (2013.01); C07K 14/50 (2013.01); C12N 5/0606 (2013.01); C12N 2501/105 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2501/12 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/48 (2013.01); C12N 2501/70 (2013.01); C12N 2501/727 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01); C12N 2509/00 (2013.01); C12N 2510/00 (2013.01);
Abstract

Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.


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