The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 24, 2020

Filed:

Oct. 03, 2017
Applicants:

President and Fellows of Harvard College, Cambridge, MA (US);

Vilnius University, Vilnius, LT;

Inventors:

David A. Weitz, Bolton, MA (US);

Allon Moshe Klein, Boston, MA (US);

Ilke Akartuna, Cambridge, MA (US);

Linas Mazutis, Vilnius, LT;

Marc W. Kirschner, Newton, MA (US);

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01J 19/00 (2006.01); B01L 3/00 (2006.01); B01F 13/00 (2006.01); C12Q 1/6806 (2018.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
B01J 19/0046 (2013.01); B01F 13/0062 (2013.01); B01L 3/502761 (2013.01); B01L 3/502776 (2013.01); B01L 3/502784 (2013.01); C12Q 1/6806 (2013.01); B01J 2219/00585 (2013.01); B01J 2219/00722 (2013.01); B01L 7/52 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/021 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0883 (2013.01);
Abstract

The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include 'barcodes' or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.


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