The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 24, 2020

Filed:

May. 20, 2016
Applicant:

Oxford Nanopore Inc., Cambridge, MA (US);

Inventors:

Ping Xie, Cambridge, MA (US);

Ken Healy, Cambridge, MA (US);

Justin Millis, Cambridge, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 67/00 (2006.01); B01D 69/02 (2006.01); C25F 7/00 (2006.01); G01N 33/487 (2006.01); B01D 69/06 (2006.01); C25F 3/02 (2006.01); B01D 69/12 (2006.01); C12Q 1/6869 (2018.01); C25F 3/14 (2006.01); B82Y 40/00 (2011.01);
U.S. Cl.
CPC ...
B01D 67/0062 (2013.01); B01D 69/02 (2013.01); B01D 69/06 (2013.01); B01D 69/12 (2013.01); C12Q 1/6869 (2013.01); C25F 3/02 (2013.01); C25F 3/14 (2013.01); C25F 7/00 (2013.01); G01N 33/48721 (2013.01); B01D 2323/42 (2013.01); B01D 2325/021 (2013.01); B01D 2325/06 (2013.01); B82Y 40/00 (2013.01);
Abstract

Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown are provided. In one disclosed arrangement a plurality of apertures are formed. The membrane comprises a first surface area portion on one side of the membrane and a second surface area portion on the other side of the membrane. Each of a plurality of target regions comprises a recess or a fluidic passage opening out into the first or second surface area portion. The method comprises contacting all of the first surface area portion of the membrane with a first bath comprising ionic solution and all of the second surface area portion with a second bath comprising ionic solution. A voltage is applied across the membrane via first and second electrodes in respective contact with the first and second baths comprising ionic solutions to form an aperture at each of a plurality of the target regions in the membrane.


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