The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 17, 2020

Filed:

Jul. 21, 2015
Applicants:

Institut National DE LA Sante ET DE LA Recherche Medicale (Inserm), Paris, FR;

Universite D'evry—val D'essonne, Evry, FR;

Inventors:

David Pastre, Evry, FR;

Michel Cailleret, Evry, FR;

Patrick Curmi, Evry, FR;

Mirela Boca, Evry, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); C12N 15/10 (2006.01); C07K 14/47 (2006.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6845 (2013.01); C07K 14/47 (2013.01); C12N 15/1055 (2013.01); G01N 33/582 (2013.01); C07K 2319/20 (2013.01); C07K 2319/30 (2013.01); C07K 2319/60 (2013.01); C07K 2319/70 (2013.01); G01N 2333/47 (2013.01);
Abstract

A method for detecting an interaction between one or more protein bait and one or more candidate prey in a eukaryotic cell, comprising the steps of: a) providing an eukaryotic cell expressing (i) one or more protein bait, and (ii) one or more candidate prey, wherein said protein bait comprises a bait moiety and a polymerized-tubulin binding moiety. b) determining the occurence of an interaction between said one or more protein bait and said one or more candidate prey in the eukaryotic cell, wherein said protein bait is bound to polymerized tubulin in the eukaryotic cell, thereby localizing said one or more candidate prey along said polymerized tubulin, thereby detecting said interaction.


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