The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 17, 2020
Filed:
Aug. 31, 2011
Fernando Decastro, Boyds, MD (US);
Renee Howell, Rockville, MD (US);
Sami Kanderian, Germantown, MD (US);
Johnathan S. Coursey, Germantown, MD (US);
Kenton C. Hasson, Germantown, MD (US);
Scott Sundberg, Rockville, MD (US);
Fernando DeCastro, Boyds, MD (US);
Renee Howell, Rockville, MD (US);
Sami Kanderian, Germantown, MD (US);
Johnathan S. Coursey, Germantown, MD (US);
Kenton C. Hasson, Germantown, MD (US);
Scott Sundberg, Rockville, MD (US);
Canon U.S.A., Inc., Melville, NY (US);
Abstract
The present invention relates to the use of one or more amplicons as temperature calibrators. In some embodiments, the calibrators may be used to calibrate the temperature of a microfluidic channel in which amplification and/or melt analysis is performed. In some embodiments, the amplicons may be genomic, ultra conserved elements and/or synthetic. The amplicon(s) may have a known or expected melt temperature(s). The calibrators may be added to primers of study or may follow or lead the primers of study in the channel. The amplicon(s) may be amplified and melted, and the temperature(s) at which the amplicon(s) melted may be determined. The measured temperature(s) may be compared to the known temperature(s) at which the amplicon(s) was expected to melt. The difference(s) between the measured and expected temperatures may be used to calibrate/adjust one or more temperature control elements used to control and/or detect the temperature of the channel.