The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 17, 2020
Filed:
Dec. 14, 2018
Applicant:
Quark Biosciences Taiwan, Inc., Hsinchu County, TW;
Inventors:
Wei-Ming Chen, Hsinchu County, TW;
Shih-Ting Kang, Hsinchu County, TW;
Assignee:
Quark Biosciences Taiwan, Inc., Hisnchu County, TW;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6853 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C12Q 1/6853 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/178 (2013.01);
Abstract
A nucleotide sequence having SEQ ID NO:1, a universal reverse primer having SEQ ID NO:2, and a universal RT primer are provided. In the miRNA detection method, the universal RT primer is used for the cDNA synthesis of a miRNA sample and the universal reverse primer is used for cDNA molecule amplification in qPCR quantitative detection. The universal reverse primer sequence, the nucleotide sequence, and the design rules are used in the method for designing primer, so as to design a primer for qPCR quantitative detection.