The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 17, 2020

Filed:

Dec. 22, 2015
Applicant:

Kyoto University, Kyoto-shi, Kyoto, JP;

Inventors:

Kenichiro Kamei, Kyoto, JP;

Momoko Honda, Kyoto, JP;

Assignee:

Kyoto University, Kyoto, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12N 15/09 (2006.01); C12N 5/073 (2010.01);
U.S. Cl.
CPC ...
C12N 5/067 (2013.01); C12M 1/00 (2013.01); C12M 3/00 (2013.01); C12N 5/0603 (2013.01); C12N 5/0676 (2013.01); C12N 15/09 (2013.01); C12N 2501/119 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/727 (2013.01); C12N 2501/999 (2013.01); C12N 2506/00 (2013.01); C12N 2506/02 (2013.01); C12N 2533/54 (2013.01); C12N 2533/90 (2013.01);
Abstract

The present invention provides an endodermal cell production method that can induce differentiation of pluripotent cells into endodermal cells even when the pluripotent cells are dispersed and can achieve improved endodermal cell production efficiency. The endodermal cell production method according to the present invention is a method for producing endodermal cells by inducing differentiation of pluripotent cells into the endodermal cells, including the step of: inducing differentiation of the pluripotent cells into the endodermal cells in the presence of an endodermal cell inducing factor. In the induction step, the cell density of the pluripotent cells at the start of the induction preferably is from 0.5×10to 2×10cells/cm.


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