The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 17, 2020

Filed:

Jul. 24, 2017
Applicant:

The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);

Inventors:

Karl Deisseroth, Stanford, CA (US);

Feng Zhang, Cambridge, MA (US);

Viviana Gradinaru, La Cañada Flintridge, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61N 5/06 (2006.01); A61K 48/00 (2006.01); A01K 67/033 (2006.01); C07K 14/195 (2006.01); C07K 14/215 (2006.01); C12N 15/86 (2006.01); C07K 14/00 (2006.01); C12N 13/00 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
A61N 5/062 (2013.01); A01K 67/0333 (2013.01); A61K 48/005 (2013.01); A61K 48/0058 (2013.01); A61K 48/0083 (2013.01); A61N 5/0613 (2013.01); A61N 5/0622 (2013.01); C07K 14/00 (2013.01); C07K 14/195 (2013.01); C07K 14/215 (2013.01); C12N 13/00 (2013.01); C12N 15/86 (2013.01); A01K 2217/052 (2013.01); A01K 2227/703 (2013.01); A01K 2267/0356 (2013.01); A61K 38/00 (2013.01); C12N 2710/10343 (2013.01); C12N 2710/16643 (2013.01); C12N 2740/16043 (2013.01); C12N 2740/16071 (2013.01); C12N 2750/14143 (2013.01); C12N 2830/002 (2013.01);
Abstract

Stimulation of target cells using light, e.g., in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated NpHR-based molecule comprising a nucleic acid sequence that codes for light-activated NpHR-based molecule and a promoter. Either a high expression of the molecule manifests a toxicity level that is less than about 75%, or the light-activated NpHR-based proteins are expressed using at least two NpHR-based molecular variants. Each of the variants characterized in being useful for expressing a light-activated NpHR-based molecule that responds to light by producing an inhibitory current to dissuade depolarization of the neuron. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion pumps or for controlling inhibitory currents in a cell (e.g., in in vivo and in vitro environments).


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