The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 10, 2020

Filed:

Oct. 04, 2013
Applicant:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Inventors:

Andrew J. Murphy, Croton-On-Hudson, NY (US);

George D. Yancopoulos, Yorktown Heights, NY (US);

Margaret Karow, Santa Rosa, CA (US);

Lynn Macdonald, White Plains, NY (US);

Sean Stevens, San Diego, CA (US);

Aris N. Economides, Tarrytown, NY (US);

David M. Valenzuela, Yorktown Heights, NY (US);

Assignee:

Rgeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A01K 67/027 (2006.01); C12P 21/00 (2006.01); C12N 15/90 (2006.01); C07K 16/00 (2006.01); C12N 15/67 (2006.01); C07K 16/46 (2006.01); C12N 15/85 (2006.01); C07K 16/28 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); A01K 67/0275 (2013.01); A01K 67/0278 (2013.01); C07K 16/00 (2013.01); C07K 16/28 (2013.01); C07K 16/462 (2013.01); C12N 15/67 (2013.01); C12N 15/85 (2013.01); C12N 15/8509 (2013.01); C12N 15/902 (2013.01); C12N 15/907 (2013.01); A01K 2207/15 (2013.01); A01K 2217/05 (2013.01); A01K 2227/105 (2013.01); A01K 2267/01 (2013.01); C07K 2317/10 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/51 (2013.01); C07K 2317/515 (2013.01); C07K 2317/56 (2013.01); C12N 2800/204 (2013.01);
Abstract

A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.


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