The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 10, 2020

Filed:

Aug. 07, 2015
Applicants:

National Center for Child Health and Development, Tokyo, JP;

Senju Pharmaceutical Co., Ltd., Osaka, JP;

Inventors:

Noriyuki Azuma, Tokyo, JP;

Taku Tanaka, Tokyo, JP;

Tadashi Yokoi, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/079 (2010.01); C12N 15/09 (2006.01); C12Q 1/02 (2006.01); A61L 27/00 (2006.01); A61P 27/06 (2006.01); A61K 35/30 (2015.01); C12Q 1/6876 (2018.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0621 (2013.01); A61K 35/30 (2013.01); A61L 27/00 (2013.01); A61P 27/06 (2018.01); C12N 15/09 (2013.01); C12Q 1/02 (2013.01); C12Q 1/6876 (2013.01); G01N 33/5058 (2013.01); C12N 2501/13 (2013.01); C12N 2506/02 (2013.01); C12N 2506/08 (2013.01); C12N 2506/45 (2013.01); C12Q 2600/136 (2013.01); C12Q 2600/158 (2013.01);
Abstract

An object of the present invention is to provide a method of inducing pluripotent stem cells to differentiate into clinically applicable retinal ganglion cells. The present invention provides a method for inducing pluripotent stem cells to differentiate into retinal ganglion cells that can be used for clinical application. Such method is a method for producing retinal ganglion cells with elongated axons comprising the following steps: (a) a step of inducing pluripotent stem cells to differentiate into retinal progenitor cells via floating culture; (b) a step of inducing the retinal progenitor cells obtained in step (a) to differentiate into retinal ganglion cells via floating culture; and (c) a step of allowing axons to elongate via adhesion culture of the retinal ganglion cells obtained in step (b).


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