The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 03, 2020
Filed:
Mar. 30, 2016
Archer Daniels Midland Company, Decatur, IL (US);
Charles A Abbas, Champaign, IL (US);
Andriy Sibirny, Lviv, UA;
Kostyantyn Dmytruk, Lviv, UA;
Barbara Kshanovska, Lviv, UA;
ARCHER DANIELS MIDLAND COMPANY, Decatur, IL (US);
Abstract
Described herein are new approaches for the selection ofstrains with increased ethanol production from hydrolyzed starch derived sugars. An industrial production strain ofAS400 was subjected to positive selection of mutants resistant to toxic concentrations of oxythiamine, trehalose, 3-bromopyruvate, glyoxylic acid, and glucosamine. The selected mutants are characterized by 5-8% increase in ethanol yield (g gof consumed glucose) as compared to the parental industrial ethanol-producing strain. A multiple-step selection approach that consisted of the sequential selection using glyoxylic acid, glucosamine and bromopyruvate as selective agents resulted in a 12% increase in ethanol yield during fermentation on industrial media. These results indicate that the selection methods provided herein are useful for producing a variety of strains that are promising candidates for industrial ethanol production.