The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 25, 2020

Filed:

Dec. 10, 2015
Applicant:

The University of Maryland, Baltimore, Baltimore, MD (US);

Inventors:

Henryk Szmacinski, Mariottsville, MD (US);

Joseph R. Lakowicz, Ellicott City, MD (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/543 (2006.01); G01N 33/553 (2006.01); G01N 21/64 (2006.01); G01N 21/27 (2006.01);
U.S. Cl.
CPC ...
G01N 33/553 (2013.01); G01N 21/648 (2013.01); G01N 21/6408 (2013.01); G01N 33/54346 (2013.01); G01N 21/274 (2013.01);
Abstract

Techniques for metal enhanced fluorescence include determining a calibration curve that relates concentration of a particular analyte to at least one of intensity or lifetime of fluorescent emissions at a plasmonic substrate in response to incident light, for a plurality of known concentrations of the particular analyte mixed with a reagent. The reagent comprises a detection molecule. A concentration of the particular analyte in a vicinity of a cell in a sample is determined directly from the calibration curve and measurements, in response to the incident light, of at least one of intensity or lifetime of fluorescent emissions at the plasmonic substrate in contact with the cell and reagent.


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