The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 25, 2020

Filed:

Mar. 11, 2016
Applicant:

The General Hospital Corporation, Boston, MA (US);

Inventors:

Conor L. Evans, Charlestown, MA (US);

W. Peter Hansen, Drury, MA (US);

Robert A. Hoffman, Livermore, CA (US);

Petra B. Krauledat, Drury, MA (US);

Daniel W. Cramer, Chestnut Hill, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); G01N 15/14 (2006.01); G01N 33/58 (2006.01); G01N 33/543 (2006.01); C12N 5/09 (2010.01); G01N 33/569 (2006.01); C12N 5/0784 (2010.01); A61K 31/7088 (2006.01); A61K 31/7105 (2006.01); C12N 15/113 (2010.01); A61K 31/713 (2006.01); G01N 15/00 (2006.01);
U.S. Cl.
CPC ...
G01N 21/648 (2013.01); C12N 5/0694 (2013.01); G01N 15/1459 (2013.01); G01N 21/6456 (2013.01); G01N 33/54346 (2013.01); G01N 33/54373 (2013.01); G01N 33/56972 (2013.01); G01N 33/582 (2013.01); G01N 33/585 (2013.01); A61K 31/7088 (2013.01); A61K 31/713 (2013.01); A61K 31/7105 (2013.01); C12N 5/064 (2013.01); C12N 15/113 (2013.01); C12N 2310/113 (2013.01); C12N 2310/141 (2013.01); G01N 2015/008 (2013.01); G01N 2015/1486 (2013.01); G01N 2333/4725 (2013.01);
Abstract

A method for detecting a target cell surface molecule and classifying cell types in a fluid sample. The method involves the addition of a reagent to the fluid sample. The reagent includes nanoparticles with optical plasmonic resonances, and at least one fluorescent probe. The nanoparticles are a bio-optical probe for the target cell surface molecule. Each fluorescent probe targets a cell classification marker. The method further involves the acquisition of an image using dark field microscopy and fluorescence microscopy to detect and quantify the presence or absence of any cells in the fluid sample having the target cell surface molecule or having the cell classification marker.


Find Patent Forward Citations

Loading…