The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 18, 2020
Filed:
May. 12, 2016
Applicant:
Seattle Children's Hospital, Seattle, WA (US);
Inventors:
Andrew Scharenberg, Seattle, WA (US);
David Rawlings, Seattle, WA (US);
Michael C. Jensen, Bainbridge Island, WA (US);
Kamila Gwiazda, Seattle, WA (US);
Alexandra Grier, Seattle, WA (US);
Assignee:
Seattle Children's Hospital, Seattle, WA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/63 (2006.01); C12N 15/90 (2006.01); C12N 15/86 (2006.01); A61K 38/46 (2006.01); A61P 35/00 (2006.01); A61K 48/00 (2006.01); C12N 7/04 (2006.01); C12N 15/113 (2010.01); C12N 15/861 (2006.01);
U.S. Cl.
CPC ...
C12N 15/907 (2013.01); A61K 38/465 (2013.01); A61K 48/005 (2013.01); A61P 35/00 (2018.01); C12N 7/045 (2013.01); C12N 15/113 (2013.01); C12N 15/86 (2013.01); C12N 15/8616 (2013.01); C12N 2310/20 (2017.05); C12N 2750/14143 (2013.01); C12N 2800/80 (2013.01);
Abstract
Disclosed herein are nuclease-based systems for genome editing and methods of using the system for genome editing. Also, disclosed are approaches to enhance Cas9-mediated gene editing efficiency in primary human cells with minimal toxicity when using adeno-associated virus vectors (AAV) to express the guide RNAs necessary for CRISPR/Cas9-based genome editing in the presence of helper proteins.