The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 11, 2020
Filed:
Aug. 30, 2017
Applicant:
Kabushiki Kaisha Toshiba, Minato-ku, JP;
Inventors:
Assignee:
KABUSHIKI KAISHA TOSHIBA, Minato-ku, JP;
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6823 (2018.01); C12Q 1/6825 (2018.01); C12Q 1/6851 (2018.01); C12N 15/09 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6837 (2013.01); C12Q 1/6823 (2013.01); C12Q 1/6825 (2013.01); C12Q 1/6851 (2013.01); C12N 15/09 (2013.01); C12Q 2525/301 (2013.01); C12Q 2527/101 (2013.01); C12Q 2537/1373 (2013.01); C12Q 2537/163 (2013.01); C12Q 2563/113 (2013.01);
Abstract
According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.