The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 11, 2020
Filed:
Mar. 29, 2017
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Liping Feng, Baltimore, MD (US);
William B. Brasso, Columbia, MD (US);
Susan M. Kircher, Hanover, PA (US);
Vanda White, Baltimore, MD (US);
Song Shi, Reisterstown, MD (US);
Xiao Mo, Ellicott City, MD (US);
Tuan-Linh Ngoc Nguyen, Carlisle, PA (US);
Adrien P. Malick, Sparks, MD (US);
Jon E. Salomon, Stewartstown, PA (US);
John D. Mantlo, Sykesville, MD (US);
BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US);
Abstract
Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.