The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 04, 2020

Filed:

May. 15, 2014
Applicant:

University of Pretoria, Pretoria, ZA;

Inventors:

Jan Adrianus Verschoor, Die Wilgers, ZA;

Carl Baumeister, Lynnwood Manor, ZA;

Assignee:

University of Pretoria, Pretoria, ZA;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/569 (2006.01); G01N 33/68 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6854 (2013.01); G01N 33/5432 (2013.01); G01N 33/5438 (2013.01); G01N 2333/35 (2013.01); G01N 2469/20 (2013.01); G01N 2800/12 (2013.01);
Abstract

Mycolic acid antigen containing liposomes, and an electrode immobilised antigens of tuberculous mycobacterial origin are produced. A diagnostic sample from a human suspected of having tuberculosis is diluted and divided into first and second samples. A control sample comprising the first sample and a redox probe is exposed to the liposomes. A test sample comprising the second sample and a redox probe is exposed to liposomes not containing mycolic acid antigens. The control sample is contacted with the immobilised antigens to allow antibodies therein to bind to the antigens. The test sample is contacted with the immobilised antigens to allow any antibodies therein to bind to the antigens. The degree of binding in the samples is compared by electrochemical impedance spectroscopy. Any observed lesser binding by the control sample is an indicator of antibodies to the antigens in the diagnostic sample, indicating tuberculosis in the human.


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