The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 04, 2020

Filed:

Feb. 27, 2017
Applicant:

Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US);

Inventors:

Alexander A. Green, Scottsdale, AZ (US);

Anli Tang, Gilbert, AZ (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6897 (2018.01); C12N 15/113 (2010.01); C12N 15/67 (2006.01); C07K 14/435 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6897 (2013.01); C07K 14/43595 (2013.01); C12N 15/113 (2013.01); C12N 15/67 (2013.01); C07K 2319/60 (2013.01);
Abstract

Provided herein is a RNA-based riboregulator that detects protein translation and uses this stimulus to regulate expression of an unrelated protein. In one embodiment, provided herein is a synthetic nucleic acid molecule configured as a translation-sensing riboregulator, where the synthetic nucleic acid molecule comprises a first nucleotide sequence encoding a polypeptide, a translation-sensing riboswitch (TSR), and a second nucleotide sequence encoding a polypeptide, wherein the translation-sensing riboswitch comprises a stop codon of the first nucleotide sequence, a fully or partially double-stranded stem-forming domain, and translation initiation elements of the second nucleotide sequence.


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