The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 04, 2020
Filed:
Mar. 07, 2014
Applicants:
Sekisui Medical Co., Ltd., Chuo-ku, JP;
Mie University, Tsu-shi, JP;
Inventors:
Takuya Yotani, Ibaraki, JP;
Tsutomu Nobori, Mie, JP;
Assignees:
SEKISUI MEDICAL CO., LTD., Chuo-ku, JP;
MIE UNIVERSITY, Tsu-shi, JP;
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6827 (2018.01); G01N 30/96 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6848 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6827 (2013.01); G01N 30/96 (2013.01);
Abstract
Provided is a rapid and simple method of detecting methylated DNA. The method of detecting methylated DNA includes the following steps of: (1) treating sample DNA with a hydrogen sulfite; (2) amplifying the sample DNA treated with the hydrogen sulfite by PCR; and (3) subjecting the resultant PCR amplification product to ion-exchange chromatography.