The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 04, 2020
Filed:
Dec. 18, 2013
Sirna Therapeutics, Inc., Cambridge, MA (US);
Daniel Zewge, Rahway, NJ (US);
Gregory T. Copeland, Claremont, CA (US);
Zhen Li, Westfield, NJ (US);
Joseph D. Armstrong, Rahway, NJ (US);
SIRNA THERAPEUTICS, INC., Cambridge, MA (US);
Abstract
This invention relates to a process for introducing two or more 2'-modifications into an RNA, wherein the RNA has a 2′-O substituent containing an alkyl ester functional group on one or more ribose rings of a strand and a 2′-O substituent containing an alkyne functional groups on one or more ribose rings on the same strand. The process comprises a) adding an amine compound to the RNA to form amidation reactions with the alkyl ester functional groups; b) dissolving the modified RNA from step (a) in a solvent to form a solution; and c) adding an organic azide and a copper or ruthenium catalyst to the solution obtained in step (b) to form 2′-azide-alkyne cycloaddition reaction products with the alkyne functional groups.