The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 04, 2020
Filed:
Dec. 24, 2015
Applicant:
Osaka University, Osaka, JP;
Inventors:
Assignee:
OSAKA UNIVERSITY, Osaka, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); A61K 38/00 (2006.01); C07K 19/00 (2006.01); C12P 21/02 (2006.01); C12N 15/09 (2006.01); C07K 14/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0018 (2013.01); A61K 38/00 (2013.01); C07K 19/00 (2013.01); C12N 15/09 (2013.01); C12P 21/02 (2013.01); C07K 14/00 (2013.01); C12N 2501/415 (2013.01);
Abstract
Provided is a novel method for producing a Wnt protein, which method comprises (1) a step of performing affinity purification targeting afamin to obtain a Wnt protein from a culture product, and/or (2) a step of performing affinity purification to obtain a Wnt protein in the form of a complex with afamin from a culture product. The method enables a Wnt protein having high Wnt activity to be produced in a simple and brief manner without use of special equipment.