The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 28, 2020
Filed:
Jul. 28, 2016
Arcis Biotechnology Holdings Limited, Warrington, GB;
ARCIS BIOTECHNOLOGY HOLDINGS LIMITED, Warrington, GB;
Abstract
A method (e.g. high resolution melting analysis) of determining of the presence or absence of a target nucleic acid in a sample, e.g. a biological sample, the method comprising steps of:⋅(a) contacting the sample with a composition comprising⋅(i) a quaternary ammonium compound, e.g. 3-(trimethoxysilyl) propyl-dimethyl octadecyl ammonium chloride (3-TPAC) or a precursor thereof, in order to release the nucleic acids from said sample; and⋅(b) contacting the sample with a probe which is capable of interacting with the nucleic acid sequence; and⋅(c) observing and/or measuring a property of the sample, e.g. fluorescence from a dsDNA binding dye.