The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 28, 2020

Filed:

Nov. 07, 2014
Applicant:

Gna Biosolutions Gmbh, Planegg/Martinsried, DE;

Inventors:

Federico Buersgens, Planegg/Martinsried, DE;

Joachim Stehr, Planegg/Martinsried, DE;

Lars Ullerich, Planegg/Martinsried, DE;

Assignee:

GNA Biosolutions GmbH, Planegg/Martinsried, DE;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6844 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6844 (2013.01); C12Q 2523/313 (2013.01); C12Q 2527/113 (2013.01); C12Q 2563/155 (2013.01);
Abstract

The invention relates to a method for the duplication of nucleic acids by means of a polymerase chain reaction, in the case of which a cycle consisting of the steps of denaturing, annealing and elongation is repeatedly performed. In one embodiment, the yield (g) of specimens of a nucleic acid to be duplicated, at the end of at least one passage of the cycle, is less than 80 percent of the specimens of the nucleic acid present at the beginning of said passage and, in the case of at least one passage of the cycle, the reaction time (tA) is less than one second. In addition, in a further embodiment, the number (k) of passages of the cycle of the polymerase chain reaction is greater than 45 and/or in at least one of the passage the cycle time tc is less than 20 seconds.


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