The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 21, 2020

Filed:

Oct. 29, 2015
Applicant:

National University Corporation Tokyo University of Agriculture and Technology, Tokyo, JP;

Inventors:

Tomoko Yoshino, Tokyo, JP;

Tsuyoshi Tanaka, Tokyo, JP;

Tadashi Matsunaga, Tokyo, JP;

Ryo Negishi, Tokyo, JP;

Hisashige Kanbara, Tokyo, JP;

Seita Nakamura, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 3/06 (2006.01); C12N 1/02 (2006.01); C12M 1/28 (2006.01); C12M 1/26 (2006.01); G01N 1/40 (2006.01);
U.S. Cl.
CPC ...
C12M 47/04 (2013.01); C12M 1/12 (2013.01); C12M 1/28 (2013.01); C12M 3/06 (2013.01); C12M 33/14 (2013.01); C12N 1/02 (2013.01); G01N 2001/4088 (2013.01);
Abstract

A cell isolation method includes: a cell trapping step of allowing a test liquid to pass through a cell trapping filter which has a plurality of through-holes in the thickness direction, thereby trapping isolation target cells contained in the test liquid on one surface of the cell trapping filter; a gel embedding step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel in which the cells are embedded, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that was hardened in the gel hardening step from the cell trapping filter.


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