The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 14, 2020

Filed:

Feb. 28, 2006
Applicants:

Masato Sato, Yokohama, JP;

Hideaki Sakai, Kawasaki, JP;

Inventors:

Masato Sato, Yokohama, JP;

Hideaki Sakai, Kawasaki, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/077 (2010.01); A61L 27/38 (2006.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0655 (2013.01); A61L 27/3817 (2013.01); A61K 35/12 (2013.01); A61L 2430/06 (2013.01); C12N 2539/10 (2013.01);
Abstract

The problem to be solved by the present invention is to provide a cultured cell sheet that expresses phenotypes of the chondroid tissue. The problems can be solved by producing the cultured cell sheet in which cells are cultured on a surface of a cell cultureware that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; bringing the cultured cell sheet in close contact with the carrier; and detaching the cultured cell sheet together with the carrier.


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