The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 07, 2020

Filed:

May. 15, 2018
Applicant:

Biomadison, Inc., Del Mar, CA (US);

Inventors:

Robert D. Fish, Tustin, CA (US);

Min Dong, Boston, MA (US);

Assignee:

BIOMADISON, INC., Del Mar, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 31/00 (2006.01); G01N 33/53 (2006.01); C12Q 1/37 (2006.01); G01N 33/542 (2006.01); G01N 33/94 (2006.01); C07K 19/00 (2006.01); C07K 14/47 (2006.01); C07K 14/00 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/37 (2013.01); C07K 14/00 (2013.01); C07K 14/47 (2013.01); C07K 19/00 (2013.01); G01N 21/6428 (2013.01); G01N 33/542 (2013.01); G01N 33/9406 (2013.01); C07K 2319/50 (2013.01); C07K 2319/60 (2013.01); G01N 2021/6432 (2013.01); G01N 2333/952 (2013.01);
Abstract

Methods for improving the specificity of assays for botulinum neurotoxins are described. Reporting constructs are provided that include cleavable linker sequence with genetically modified recognition and/or cleavage sites. These linker sequences act as a substrate for a botulinum neurotoxin, with cleavage of the reporting construct providing a detectable signal. When first and second neurotoxins have activity with the same substrate protein, mutation and/or deletion of a recognition and/or cleavage site associated with the second neurotoxin improves the specificity of the detectable signal for the first neurotoxin.


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