The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 07, 2020
Filed:
Sep. 18, 2014
Applicant:
The General Hospital Corporation, Boston, MA (US);
Inventors:
Shengdar Tsai, Charlestown, MA (US);
J. Keith Joung, Winchester, MA (US);
Assignee:
The General Hospital Corporation, Boston, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); C12P 19/34 (2006.01); C07K 14/47 (2006.01); C12N 15/63 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C07K 14/4705 (2013.01); C12N 15/1136 (2013.01); C12N 15/63 (2013.01); C07K 2319/40 (2013.01); C07K 2319/43 (2013.01); C07K 2319/80 (2013.01); C12N 2310/20 (2017.05); C12N 2310/318 (2013.01); C12N 2310/3231 (2013.01); C12N 2310/3341 (2013.01);
Abstract
Methods and constructs for the multiplex expression of highly active CRISPR guide RNAs (gRNAs) from RNA Polymerase II and III promoters, optionally in mammalian cells. The present invention is based, at least in part, on the discovery that Csy4, an endoribonuclease that recognizes a short RNA hairpin sequence, can be used to cleave out multiple functional gRNAs encoded on a single longer RNA transcript (produced from an RNA pol II or III promoter) in which the individual gRNAs are separated by Csy4 cleavage sites.