The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 07, 2020

Filed:

Jul. 22, 2015
Applicants:

The University of Notre Dame Du Lac, Notre Dame, IN (US);

University of Massachusetts, Boston, MA (US);

Inventors:

Brian Baker, Granger, IN (US);

Lance Hellman, Granger, IN (US);

Brian Pierce, Darnestown, MD (US);

Zhiping Weng, Wellesley, MA (US);

Assignees:

The University of Notre Dame du Lac, Notre Dame, IN (US);

University of Massachusetts, Boston, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/725 (2006.01); C12N 5/0783 (2010.01); C12N 15/86 (2006.01); A61K 35/17 (2015.01); A61K 38/17 (2006.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
C07K 14/7051 (2013.01); A61K 35/17 (2013.01); A61K 38/1774 (2013.01); C12N 5/0636 (2013.01); C12N 15/86 (2013.01); A61K 2035/124 (2013.01); C12N 2510/00 (2013.01);
Abstract

Molecular constructs and dual recognition constructs having a sequence encoding a TCR affinity weakening motif, and DNA and RNA sequences corresponding thereto, are presented. Modified T-cells and other cells transformed with the molecular contracts express a modified TCR that imparts a reduction, in non-specific binding, -an enhancement of binding specificity and an enhancement of binding affinity for a target antigen, compared to non-transformed (wild-type, native) T-cells, are described. The modified TCRs possess an affinity enhancing motif and an affinity weakening motif. Methods of transforming cells and methods of using enriched populations of transformed cells, in the treatment of cancer and infections arid T-cell mediated pathologies are provided. The affinity weakening motif imparts a weakened interaction, of a TCR with major histocompatibility complex proteins, such as HLA proteins in humans. Soluble modified TCRs are also provided, Therapeutic preparations comprising modified T-cells, modified TCRs, and modified TCR-therapeutic agent-conjugates, are also provided.


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