The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 31, 2019
Filed:
Feb. 13, 2018
City of Hope, Duarte, CA (US);
Markus Kalkum, Azusa, CA (US);
Ismail Al-Abdullah, Monrovia, CA (US);
CITY OF HOPE, Duarte, CA (US);
Abstract
Provided herein are novel fluorogenic and bioluminescent bacterial protease substrates that contain at least one bacterial protease cleavage site. In some embodiments, the proteases that cleave the protease substrates are bacterial proteases including those used for tissue dissociation, cell isolation, and cell detachment, such as the preparation of islet cells. Additionally, the novel protease substrates described herein may be used to assess the activity of certain bacterial proteases involved in isolating primary cells and stem cells. Methods and protease detection kits using the novel protease substrates are also provided herein.