The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 31, 2019
Filed:
Oct. 13, 2014
Servicio Galego DE Saúde (Sergas), Santiago de Compostela, ES;
Fundación Profesor Novoa Santos, A Coruña, ES;
Germán Bou Arévalo, A Coruña, ES;
María Clara Póvoa Cabral, A Coruña, ES;
Astrid Pérez Gómez, A Coruña, ES;
María Merino Carballeira, A Coruña, ES;
Alejandro Beceiro Casas, A Coruña, ES;
Patricia García Fernández, A Coruña, ES;
SERVICIO GALEGO DE SAÚDE (SERGAS), Santiago de Compostela, ES;
FUNDACIÓN PROFESOR NOVOA SANTOS, A Coruña, ES;
Abstract
The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wail, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.