The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 24, 2019

Filed:

Apr. 09, 2015
Applicant:

The Scripps Research Institute, La Jolla, CA (US);

Inventors:

Floyd E. Romesberg, La Jolla, CA (US);

Denis A. Malyshev, La Jolla, CA (US);

Assignee:

The Scripps Research Institute, La Jolla, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/70 (2006.01); C12P 19/34 (2006.01); C07K 14/295 (2006.01); C07K 14/405 (2006.01); C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
C12N 15/70 (2013.01); C07K 14/295 (2013.01); C07K 14/405 (2013.01);
Abstract

A recombinantly expressed nucleotide triphosphate transporter efficiently imports the triphosphates of unnatural nucleotides into cells, and the endogenous cellular machinery incorporates those nucleotides into cellular nucleic acids. UBPs can therefore form within the cell's nucleic acids. Moreover, neither the presence of the unnatural triphosphates nor the replication of the UBP represents a significant growth burden. The UBP is not efficiently excised by nucleic acid repair pathways, and therefore can be retained as long as the unnatural triphosphates are available in the growth medium. Thus, the resulting cell is the first organism to stably propagate an expanded genetic alphabet.


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