The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 24, 2019
Filed:
Mar. 23, 2011
Yoko Itchoda, Ibaraki, JP;
Go Tazaki, Ibaraki, JP;
Masaya Hosoda, Ibaraki, JP;
Motohiro Fukuda, Ibaraki, JP;
Hideki Taniguchi, Kanagawa, JP;
Yun-wen Zheng, Kanagawa, JP;
Keisuke Sekine, Kanagawa, JP;
Yoko Itchoda, Ibaraki, JP;
Go Tazaki, Ibaraki, JP;
Masaya Hosoda, Ibaraki, JP;
Motohiro Fukuda, Ibaraki, JP;
Hideki Taniguchi, Kanagawa, JP;
Yun-Wen Zheng, Kanagawa, JP;
Keisuke Sekine, Kanagawa, JP;
CORNING INCORPORATED, Corning, NY (US);
PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY, Yokohama-Shi, Kanagawa, JP;
Abstract
Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber () having a plurality of microchambers () formed on a culture surface. The cell culture chamber () has a culture surface formed of spaces in which the microchambers () have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μmto 1 mm. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers ().