The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 17, 2019

Filed:

Oct. 25, 2017
Applicant:

The University of British Columbia, Vancouver, CA;

Inventors:

Carl Lars Genghis Hansen, Vancouver, CA;

Michael Vanlnsberghe, Vancouver, CA;

Adam White, Toronto, CA;

Oleh Petriv, Vancouver, CA;

Tim Leaver, Vancouver, CA;

Anupam Singhal, Mississauga, CA;

William Bowden, Vancouver, CA;

Veronique Lecault, Vancouver, CA;

Dan Da Costa, Vancouver, CA;

Leo Wu, Surrey, CA;

Georgia Russell, Vancouver, CA;

Darek Sikorski, Vancouver, CA;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/543 (2006.01); C12Q 1/6806 (2018.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01); C12M 3/06 (2006.01); B01L 3/00 (2006.01); G01N 1/34 (2006.01); C12Q 1/6844 (2018.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); B01L 3/502753 (2013.01); B01L 3/502761 (2013.01); C12M 23/16 (2013.01); G01N 1/34 (2013.01); G01N 33/5005 (2013.01); G01N 33/54366 (2013.01); G01N 33/569 (2013.01); B01L 7/52 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0816 (2013.01); B01L 2400/086 (2013.01); C12Q 1/6844 (2013.01);
Abstract

Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.


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