The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 17, 2019
Filed:
Oct. 02, 2015
Applicant:
President and Fellows of Harvard College, Cambridge, MA (US);
Inventors:
David R. Liu, Lexington, MA (US);
Vikram Pattanayak, Cambridge, MA (US);
Assignee:
President and Fellows of Harvard College, Cambridge, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/44 (2006.01); C12N 9/22 (2006.01); C12Q 1/6874 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6869 (2018.01); A61K 38/46 (2006.01); C12Q 1/6883 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/44 (2013.01); A61K 38/465 (2013.01); C12N 9/22 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6883 (2013.01); C12Y 301/00 (2013.01); C12Q 2600/156 (2013.01);
Abstract
Some aspects of this disclosure provide strategies, methods, and reagents for determining nuclease target site preferences and specificity of site-specific endonucleases. Some methods provided herein utilize a novel 'one-cut' strategy for screening a library of concatemers comprising repeat units of candidate nuclease target sites and constant insert regions to identify library members that can been cut by a nuclease of interest via sequencing of an intact target site adjacent and identical to a cut target site.