The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 17, 2019

Filed:

Aug. 01, 2014
Applicant:

Two Cells Co., Ltd., Hiroshima-shi, Hiroshima, JP;

Inventors:

Koichiro Tsuji, Hiroshima, JP;

Minoru Morikawa, Hiroshima, JP;

Satoshi Maeda, Hiroshima, JP;

Yui Kitayama, Hiroshima, JP;

Aki Ohmori, Hiroshima, JP;

Jin Chang Shao, Hiroshima, JP;

Shinichi Hasegawa, Hiroshima, JP;

Yukio Kato, Hiroshima, JP;

Takashi Matsushita, Tokyo, JP;

Masato Takao, Tokyo, JP;

Shinya Miki, Tokyo, JP;

Ken Innami, Tokyo, JP;

Yasufumi Takahashi, Tokyo, JP;

Assignee:

TWO CELLS Co., Ltd., Hiroshima, JP;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61L 27/38 (2006.01); A61L 27/36 (2006.01); A61K 35/28 (2015.01); C12N 5/077 (2010.01); A61L 27/54 (2006.01); A01N 1/02 (2006.01); C12N 5/0775 (2010.01); A61K 35/32 (2015.01);
U.S. Cl.
CPC ...
A61L 27/3895 (2013.01); A01N 1/0284 (2013.01); A61K 35/28 (2013.01); A61L 27/3654 (2013.01); A61L 27/3834 (2013.01); A61L 27/3852 (2013.01); A61L 27/54 (2013.01); C12N 5/0655 (2013.01); C12N 5/0662 (2013.01); A61K 35/32 (2013.01); A61L 2300/412 (2013.01); A61L 2300/414 (2013.01); A61L 2430/06 (2013.01); C12N 2501/10 (2013.01); C12N 2501/11 (2013.01); C12N 2501/12 (2013.01); C12N 2501/135 (2013.01); C12N 2501/15 (2013.01);
Abstract

A method according to the present invention for producing a cartilage-damage treatment agent, includes the steps of: (i) proliferating mesenchymal stem cells in a serum-free medium A containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid and at least one fatty acid; and (ii) mixing the mesenchymal stem cells thus proliferated in the step (i), an isotonic preserving agent, and a cytoprotective agent. This method provides a cartilage-damage treatment agent which favorably regenerates a cartilage tissue.


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