The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 10, 2019
Filed:
Feb. 19, 2018
Pcr amplification methods for detecting and quantifying sulfate-reducing bacteria in oilfield fluids
Applicant:
Baker Hughes, a GE Company, Llc, Houston, TX (US);
Inventor:
Crystal Lee, Houston, TX (US);
Assignee:
Baker Hughes, a GE company, LLC, Houston, TX (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C09K 8/532 (2006.01); C09K 8/02 (2006.01); C10G 31/00 (2006.01); C09K 8/58 (2006.01); C09K 8/62 (2006.01); C12Q 1/686 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C09K 8/02 (2013.01); C09K 8/58 (2013.01); C09K 8/62 (2013.01); C10G 31/00 (2013.01);
Abstract
At least one nucleic acid from a sulfate-reducing bacteria (SRB) may be extracted from an oilfield fluid and may be amplified by a PCR amplification method in the presence of at least one primer to form an amplification product. The primer(s) may be or include a sequence including, but not necessarily limited to, SEQ ID NO: 20, SEQ ID NO: 21, and mixtures thereof. The amplification product may be hybridized with a probe specific for a fragment of an alpha subunit of an APS gene, and a presence of hybridization and a degree of hybridization may be detected.