The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 03, 2019
Filed:
Oct. 14, 2014
Mgi Tech Co., Ltd., Guangdong, CN;
Yuan Jiang, Shenzhen, CN;
Chunyu Geng, Shenzhen, CN;
Xia Zhao, Shenzhen, CN;
Shujin Fu, Shenzhen, CN;
Lingyu He, Shenzhen, CN;
Yaqiao Li, Shenzhen, CN;
Xiaoshan Su, Shenzhen, CN;
Fanzi Wu, Shenzhen, CN;
Wenwei Zhang, Shenzhen, CN;
Hui Jiang, Shenzhen, CN;
Andrei Alexeev, Woodland, CA (US);
Radoje Drmanac, Mountain View, CA (US);
MGI TECH CO., LTD., , CN;
Abstract
Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3′ end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3′ end thereof can be in a complementary pairing with the 5′ end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.