The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 03, 2019

Filed:

Jan. 29, 2016
Applicant:

The Johns Hopkins University, Baltimore, MD (US);

Inventors:

Florin M. Selaru, Baltimore, MD (US);

Ling Li, Baltimore, MD (US);

Stephen J. Gould, Baltimore, MD (US);

Assignee:

THE JOHNS HOPKINS UNIVERSITY, Baltimore, MD (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); A61K 48/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12P 21/00 (2006.01); A61K 31/7105 (2006.01); C12P 1/00 (2006.01); A61K 9/107 (2006.01); A61K 31/685 (2006.01); A61K 31/704 (2006.01); A61K 33/24 (2019.01); A61K 45/06 (2006.01); C12P 19/34 (2006.01); G01N 33/574 (2006.01); A61K 47/69 (2017.01); A61K 35/13 (2015.01); A61K 35/33 (2015.01); A61P 25/00 (2006.01); C12N 15/113 (2010.01); A61K 33/243 (2019.01); A61K 9/50 (2006.01);
U.S. Cl.
CPC ...
A61K 48/0008 (2013.01); A61K 9/1075 (2013.01); A61K 31/685 (2013.01); A61K 31/704 (2013.01); A61K 31/7105 (2013.01); A61K 33/24 (2013.01); A61K 33/243 (2019.01); A61K 35/13 (2013.01); A61K 35/33 (2013.01); A61K 45/06 (2013.01); A61K 47/6901 (2017.08); A61K 48/0075 (2013.01); A61K 48/0091 (2013.01); A61P 25/00 (2018.01); C12N 15/113 (2013.01); C12P 1/00 (2013.01); C12P 19/34 (2013.01); C12P 21/00 (2013.01); G01N 33/574 (2013.01); G01N 33/57438 (2013.01); A61K 9/5068 (2013.01); C12N 2310/141 (2013.01); C12N 2320/32 (2013.01);
Abstract

The present invention relates to the field of extracellular vesicles. More specifically, the present invention provides methods and compositions for using extracellular vesicles as a vector for nucleic acid treatment in vivo of various diseases. In a specific embodiment, the present invention provides an extracellular vesicle isolated from a cell comprising one or more microRNAs (miRNAs) that have been loaded ex vivo into the vesicle so that the miRNAs are present in a higher concentration than when measured in the same extracellular vesicle isolated directly from the cell. In another embodiment, the present invention provides a method for treating cholangiocarcinoma in a subject comprising the step of administering to the subject a plurality of exosomes comprising miR-195.


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