Method and device for optical detection of a movement in a biological sample with a spatial extent

Abstract

The invention relates to a method and a device for optical in vitro detection of a movement in a biological sample with a spatial extent in the form of a three-dimensional cell and/or tissue culture or a cell cluster or a sample made of freely swimming microorganisms. The method comprises the following steps: (a) providing a receptacle for the sample (), a light beam source (), an optical unit () and a detector (), (a1) wherein the optical unit () is embodied to illuminate the whole sample () in the receptacle with radiation emanating from the light beam source and to guide at least some of the radiation () of the light beam source (), which is changed at any point within the sample () by an interaction with the sample () in terms of the beam direction thereof, the polarization state thereof and/or the diffraction pattern thereof, to a detection surface () of the detector (), and (a2) wherein the detector () is embodied to generate a measurement signal () in a manner dependent on the detected radiation, the time profile of which measurement signal specifies a time profile of an intensity of the detected radiation () and/or from which the time profile of the intensity of the detected radiation () is derivable; (b) illuminating the sample () with radiation from the light beam source; and (c) detecting a movement in the biological sample () in a manner dependent on a temporal change in the measurement signal ().