The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 26, 2019
Filed:
Mar. 23, 2017
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
Nicole Nichols, Reading, MA (US);
Gregory Patton, Peabody, MA (US);
Janine Graham, Beverly, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/16 (2006.01); C12N 9/12 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); C12N 15/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/16 (2013.01); C12N 9/1252 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6848 (2013.01); C12Y 207/07007 (2013.01); C12Y 301/00 (2013.01);
Abstract
A probe qPCR master mix is provided. In some embodiments, the master mix comprises nucleotides, an enzyme comprising a polymerase activity and a flap endonuclease activity, a chelating agent at a concentration greater than 5 μM, and a divalent cation. The relatively high concentration of chelating agent stabilizes the flap endonuclease activity during storage. As such, the polymerase and flap endonuclease activities may be substantially the same before and after storing the master mix for 7 days at 37° C.