The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 19, 2019

Filed:

Oct. 27, 2015
Applicant:

The Governing Council of the University of Toronto, Toronto, CA;

Inventors:

Craig Simmons, Toronto, CA;

Oleg Chebotarev, Toronto, CA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/00 (2006.01); G01N 33/50 (2006.01); C12M 3/06 (2006.01); C12N 5/00 (2006.01); B01L 3/00 (2006.01); C12M 1/32 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5088 (2013.01); B01L 3/5027 (2013.01); B01L 3/5085 (2013.01); C12M 23/12 (2013.01); C12M 23/16 (2013.01); C12M 23/40 (2013.01); C12M 25/02 (2013.01); C12M 29/10 (2013.01); C12N 5/0075 (2013.01); G01N 33/5058 (2013.01); G01N 33/5067 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0829 (2013.01); B01L 2400/049 (2013.01); G01N 2500/10 (2013.01);
Abstract

A microfluidic device, method and kit for assaying and/or culturing cells are provided. The microfluidic device comprises a well block comprising a plurality of microwells; at least one cell culture layer selected from a first cell culture layer comprising a plurality of microchannels, each microchannel being aligned with one of the plurality of microwells and being in fluid communication with the aligned microwells; and a second cell culture layer comprising a plurality of cell culture chamber wells, each cell culture chamber well being aligned with one of the plurality of microwells and being in fluid communication with the aligned microwells, and a plurality of outlets, each of the plurality of outlets corresponding to one of the plurality of cell culture chamber wells; and a base block, wherein the at least one cell culture layer is sealably coupled between the well block and the base block, thereby allowing fluid communication between the plurality of microwells in the well block and the at least one cell culture layer.


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