The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 19, 2019
Filed:
Jun. 07, 2011
Janina Kirchhoff, Aachen, DE;
Stefan Schillberg, Aachen, DE;
Andreas Schiermeyer, Köln, DE;
Helga Schinkel, Aachen, DE;
Rainer Fischer, Aachen, DE;
Janina Kirchhoff, Aachen, DE;
Stefan Schillberg, Aachen, DE;
Andreas Schiermeyer, Köln, DE;
Helga Schinkel, Aachen, DE;
Rainer Fischer, Aachen, DE;
Abstract
The invention provides a method for the generation of a monoclonal plant cell line from a heterologous population of plant cells, comprising the following steps: (a) provision of a heterologous population of plant cells; (b) preparation of protoplasts from said heterologous population of plant cells; (c) separation of single protoplasts by subjecting the preparation of protoplasts to flow cytometric sorting; (d) regeneration of a separated single transformed protoplast until the formation of a microcolony by co-cultivation in the presence of feeder cell material; (e) removal of the microcolony from the feeder cell material and cultivation of the microcolony until the formation of a monoclonal plant cell line.