In vitro culturing or expanding human or animal tissue

Abstract

The invention relates to a method of in vitro culturing or expanding human or animal tissue ('). The method comprises: obtaining a sample of human or animal tissue; downsizing tissue (′) of the sample; generating an assembly by placing the downsized tissue on a scaffold or hydrogel (′); arranging the assembly inside a culture chamber of a 3D perfusion system (′); and perfusing the assembly in the 3D perfusion system for a predefined time (′). The method according to the invention allows for preparing and directly culturing in vitro fresh tissue specimens for a high efficient in vitro culturing using a perfused bioreactor system. As single cells of tissue are in most cases undergoing to an in vitro cell-death due to missing micro environmental signals, the use of downsized tissue, comprising cell clumps and tissue fragments of hundreds of cells, may help to prevent cell death in vitro as well as to maintain the initial heterogeneous tissue microenvironment consisting of specific cell types such as epithelial, stromal and immune cells together. Thus, the method according to the invention is capable of efficiently culturing the whole tissue over time, thus allowing for survival of original cell types.