The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 12, 2019

Filed:

Mar. 26, 2015
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Ting-Hsiang S. Wu, Culver City, CA (US);

Pei-Yu E. Chiou, Los Angeles, CA (US);

Michael A. Teitell, Tarzana, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/89 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 1/42 (2006.01); C12N 15/87 (2006.01);
U.S. Cl.
CPC ...
C12N 15/89 (2013.01); C12M 25/02 (2013.01); C12M 33/04 (2013.01); C12M 35/00 (2013.01); C12M 35/04 (2013.01); C12N 15/87 (2013.01);
Abstract

In various embodiments, method and devices for delivering large cargos (e.g., organelles, chromosomes, bacteria, and the like) into cells are provided. In certain embodiments method of delivering a large cargo into eukaryotic cells, are provided that involve providing eukaryotic cells disposed on one side of a porous membrane; providing the cargo to be delivered in a solution disposed in a reservoir chamber on the opposite side of the porous membrane; and applying pressure to the reservoir chamber sufficient to pass the cargo through pores comprising said porous membrane wherein said cargo passes through cell membranes and into the cells.


Find Patent Forward Citations

Loading…