The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 12, 2019
Filed:
May. 20, 2015
Applicant:
Kyoto University, Kyoto, JP;
Inventors:
Kenji Osafune, Kyoto, JP;
Taro Toyoda, Kyoto, JP;
Assignee:
KYOTO UNIVERSITY, Kyoto, JP;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); A61K 35/39 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0676 (2013.01); A61K 35/39 (2013.01); C12N 2501/11 (2013.01); C12N 2501/117 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/41 (2013.01); C12N 2501/727 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); Y02A 20/402 (2018.01);
Abstract
Provided is a method for generating pancreatic bud cells, having the step of culturing PDX1/NKX6.1cells in a medium containing KGF, EGF and a BMP inhibitor. The culturing step may be performed in suspension cultures or in adherent cultures. When the cells are cultured in adherent cultures, the cells may be cultured in a medium further containing a ROCK inhibitor or a nonmuscle myosin II inhibitor.