The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 12, 2019

Filed:

Aug. 18, 2014
Applicant:

The University of Tokyo, Tokyo, JP;

Inventors:

Hiroyuki Noji, Tokyo, JP;

Rikiya Watanabe, Tokyo, JP;

Hiroaki Suga, Tokyo, JP;

Daishi Fujita, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01J 19/00 (2006.01); G01N 33/487 (2006.01);
U.S. Cl.
CPC ...
B01L 3/5085 (2013.01); B01J 19/0046 (2013.01); B01L 3/502769 (2013.01); G01N 33/48721 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00331 (2013.01); B01J 2219/00662 (2013.01); B01J 2219/00725 (2013.01); B01J 2219/00734 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/0896 (2013.01); B01L 2300/165 (2013.01);
Abstract

A material film is formed as a thin film having a thickness of 1 μm on a surface of a glass substrate. A plurality of micro-chambers having a diameter of 5 μm are formed in the material film to be arrayed at a high density. The respective chambers filled with an aqueous test solution have openings that are liquid-sealed by a lipid bilayer membrane to provide a high-density micro-chamber array. Significant downsizing of the micro-chambers enhances a change in concentration by a reaction of one biomolecule in the chamber and thereby increases the detection sensitivity. In the configuration that a large number of micro-chambers are formed at a high density, even in the case of an extremely slow reaction of the biomolecule, the reaction proceeds in any of the chambers. This configuration accordingly enables the reaction of the biomolecule to be detected with high sensitivity.


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