The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 05, 2019

Filed:

Sep. 07, 2017
Applicants:

Achira Labs Pvt. Ltd., Bangalore, IN;

Vasan Satya Srini, Bangalore, IN;

Inventors:

Dhananjay Dendukuri, Bangalore, IN;

Satish Kalme, Bangalore, IN;

Vijetha Nagendra Prakash, Bangalore, IN;

Gokul R Prasath, Bangalore, IN;

Vasan Satya Srini, Bangalore, IN;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/076 (2010.01); C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0612 (2013.01); C12N 5/061 (2013.01); C12N 2531/00 (2013.01); C12N 2533/80 (2013.01); C12N 2537/00 (2013.01);
Abstract

The method provides a hyaluronic-acid binding based technique to separate functionally competent and mature sperm. The method for extracting viable sperms from a semen sample comprises incubating functionalized HA with functionalized paramagnetic beads to obtain a plurality of HA coated paramagnetic beads. The method further comprises incubating the plurality of HA coated paramagnetic beads with the semen sample to obtain a first population and a second population of sperm. The first population comprises sperm bound to the plurality of HA coated paramagnetic beads and the second population comprises sperms not bound to the plurality of HA coated paramagnetic beads. The method comprises magnetically separating the first population from the second population and separating sperms from the plurality of HA coated paramagnetic beads in the separated first population to obtain the viable sperms.


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