The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 29, 2019

Filed:

May. 31, 2013
Applicant:

Heptares Therapeutics Limited, Cambridge, GB;

Inventors:

Fiona Hamilton Marshall, Cambridge, GB;

Seyed Ali Jazayeri-Dezfuly, Cambridge, GB;

Jayesh Chhotubhai Patel, Cambridge, GB;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 33/68 (2006.01); C07K 14/72 (2006.01); G01N 33/542 (2006.01); G01N 33/566 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6842 (2013.01); C07K 14/723 (2013.01); G01N 21/6428 (2013.01); G01N 33/542 (2013.01); G01N 33/566 (2013.01); G01N 2333/705 (2013.01); G01N 2458/00 (2013.01);
Abstract

The invention provides an assay for assessing the conformational stability of a membrane protein, comprising: (a) providing a sample comprising a first population and a second population of a membrane protein; wherein the membrane protein in the first population is labelled with a donor label and the membrane protein in the second population is labelled with an acceptor label, or the membrane protein in the first population is labelled with an acceptor label and the membrane protein in the second population is labelled with a donor label, (b) exposing the first and second populations of the membrane protein to a stability modulating agent and/or condition, (c) and assessing aggregation between membrane proteins of the first and second populations by activating the donor label to permit a distance-dependent interaction with the acceptor label, which interaction produces a detectable signal.


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