The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 29, 2019

Filed:

Oct. 31, 2017
Applicant:

Conagen Inc., Bedford, MA (US);

Inventor:

Nicky C. Caiazza, Rancho Santa Fe, CA (US);

Assignee:

Conagen Inc., Bedford, MA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/705 (2006.01); C12N 1/00 (2006.01); C12N 9/24 (2006.01); C12N 15/79 (2006.01); C12P 21/00 (2006.01); C07K 16/32 (2006.01); C12N 9/10 (2006.01);
U.S. Cl.
CPC ...
C12P 21/005 (2013.01); C07K 16/32 (2013.01); C12N 9/1051 (2013.01); C12N 15/79 (2013.01); C12Y 204/01258 (2013.01); C07K 2317/14 (2013.01); C07K 2317/41 (2013.01);
Abstract

The present invention provides recombinant cells that contain a modification causing altered expression or function of at least one mannosyl transferase enzyme. As a result of the modification the cells produce a glycoprotein or glycopeptide that has an N-linked glycan profile that is simplified or humanized. The glycoprotein or glycopeptide can have at least 25% fewer high mannose structures on than the glycoprotein or glycopeptide produced by a cell that does not have the modification. In some embodiments the modification is a deletion, knock out, or disruption of a gene encoding a mannosyl transferase, which can be in an Alg3 gene. Therefore, the proteins produced avoid many of the problems associated with the therapeutic use of glycoproteins from species having foreign or plant-like patterns of glycosylation. The invention also provides compositions of the glycoproteins or glycopeptides and methods of making them.


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